Items 1-12 of 99 Results
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Macrophage polarization: plasticity in health and disease
Learn more about generating the whole macrophage spectrum in vitro and their role in health and disease. -
Macrophages Subtypes: Polarization, Activation and Plasticity
Macrophages are multitasking cells at the first line of defense against pathogens invasion. But they can also play a major role in destroying tissues and developing cancer, autoimmune disorder or allergies. -
Cells in action: Differentiation of human mesenchymal stem cells into neurons
Cells in action: Differentiation of human mesenchymal stem cells into neurons In this video we can see the differentiation of PromoCell umbilical cord mesenchymal stem cells into neurons. Excitingly, to our knowledge this is one of the first high-resolution, long-term, live cell time-lapses showing this process. These cells were grown for 13 days in PromoCell complete mesenchymal stem cell neurogenic differentiation medium prior to capture of this film. Images were captured every 30 seconds over a 20-hour period using NanoLive’s 3D Cell Explorer. -
Cells in Action: membrane protrusions in HUVECs (Human Umbilical Vein Endothelial Cells)
During angiogenesis, cell-matrix interactions are crucial. The cell matrix structures are involved in migration, invasion and survival of cells throughout the angiogenic process. In this video we can see filopodia and lamellipodia in great detail, thanks to Nanolive imaging's ability to view membrane deformations. These structures are essential for migration, cell-cell interactions, sensing of the cell environment and more. While filopodia appear as long, thin protrusion-like structures that emerge from the cellular membrane, lamellipodia are broad, sheet-shaped structures containing thin and short interconnected actin filaments. -
Cells in Action: Double mitosis of Human Umbilical Vein Endothelial Cells (HUVECs)
Images of cells undergoing mitosis are mind-blowing, and non more beautiful than those of our Human Umbilical Vein Endothelial Cells (HUVECs) captured by Nanolive's 3D Cell Explorer. On the right hand side, the cell seems to enter mitosis but the chromatids don't complete segregation and the cell returns to interphase without dividing. Exiting mitosis is controlled by proteolysis and cyclin dependent kinases (CDKs). Mitosis regulatory machinery sometimes detects errors and forces a return to interphase, as seen here. This kind of research helps develop understanding of the cell cycle, which can be used in myriad applications including cancer research. -
Cells in Action: Mitosis in Human Mesenchymal Stem Cells (MSCs)
Watch the process of cell division in PromoCell's human mesenchymal stem cells! These images were captured using Nanolive’s non-invasive technology, which allows us to access the dynamics of biological processes, such as mitosis, with unprecedented resolution. -
Macrophage Cell Culture
Discover PromoCell’s macrophage cell culture portfolio. This ranges from a media system for generating macrophages to assay-ready cryopreserved macrophages, as well as essential macrophage analysis tools. -
Cells in Action: Human Primary Keratinocytes at the stratum granulosum
Keratinocytes at the stratum granulosum contain two characteristic structures that are visible in this high resolution footage by Nanolive - the lamellar bodies and keratohyalin granules. While keratohyalin granules contain proteins involved in the aggregation of keratin filaments and in the formation of the cell envelope, lamellar bodies contain lipids and fuse with the plasma membrane in order to secrete the content into the extracellular space. -
Differentiation of M1- or M2-Macrophages from PBMC/Monocytes
Here we describe how the presence of PromoCell differentiation media encourages in vitro differentiation of mononcytes to macrophages. These then show either an M1-like or an M2-like polarization. -
Holotomography-Monitored Phagocytosis Assay of M1 Macrophages
Phagocytosis is an essential process for pathogen clearance and is the subject of research for many immunologists. Find out more about PromoCell's phagocytosis assay for fluorescence microscopy and how you can implement this method in your lab. -
Convenient Large-Scale Differentiation of Fully Customized Monocyte-Derived Macrophages From Human PBMC In Vitro
Primary human macrophages are difficult to isolate and do not proliferate in culture. PromoCell offers a complete system for easy and cost-efficient generation of pure monocyte-derived macrophages. -
Standardized culture of assay-ready and fully functional human primary macrophages
Cryopreserved human macrophages are now available as a reliable source of standardized cells, and our ready-to-use format provides full experimental flexibility. With this easy-to-handle application, your macrophage research will be more efficient.